A cost-effective straightforward protocol for shotgun Illumina libraries designed to assemble complete mitogenomes from non-model species

Marie-Ka Tilak 1 Fabienne Justy 1 Mélanie Debiais-Thibaud 1 Fidel Botero-Castro 1 Frédéric Delsuc 1 Emmanuel Douzery 1
1 ISEM - Institut des Sciences de l'Evolution de Montpellier
Abstract : The mitogenome is an inescapable tool in conservation biology studies. Yet, its routine sequencing may remain tricky despite next-generation sequencing technologies. An enrichment step is often necessary but not always straightforward depending on the initial DNA quality or quantity. Furthermore, the availability of close mitochondrial DNA reference sequences for non-model species limits the primer design for long-range PCR or bait synthesis. Here we propose an easy and cost-effective protocol without enrichment step for building and sequencing multiplexed Illumina libraries from small quantities of either high-quality or degraded genomic DNA. We validated the approach through the successful assembly of the complete mitogenome of 60 bats and 7 tunicates. Our protocol allows the sequencing and assembly of mitochondrial genomes from non-model species with sufficient coverage for applications in conservation genetics.
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Article dans une revue
Conservation Genetics Resources, Springer, 2015, 7, pp.37 - 40
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https://hal.archives-ouvertes.fr/hal-01925895
Contributeur : Frederic Delsuc <>
Soumis le : jeudi 29 novembre 2018 - 13:23:02
Dernière modification le : samedi 1 décembre 2018 - 01:21:46

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Marie-Ka Tilak, Fabienne Justy, Mélanie Debiais-Thibaud, Fidel Botero-Castro, Frédéric Delsuc, et al.. A cost-effective straightforward protocol for shotgun Illumina libraries designed to assemble complete mitogenomes from non-model species. Conservation Genetics Resources, Springer, 2015, 7, pp.37 - 40. 〈hal-01925895〉

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