Use of fluorescence in situ hybridization as a tool for introgression analysis and chromosome identification in coffee (Coffea arabica L.)

Abstract : Fluorescence in situ hybridization (FISH) was used to study the presence of alien chromatin in interspecific hybrids and one introgressed line (S.288) derived from crosses between the cultivated species Coffea arabica and the diploid relatives C canephora and C. liberica. In situ hybridization using genomic DNA from C. canephora and C. arabica as probes showed elevated cross hybridization along the hybrid genome, confirming the weak differentiation between parental genomes. According to our genomic in situ hybridization (GISH) data, the observed genomic resemblance between the modern C. canephora genome (C) and the C. canephora-derived subgenome of C. arabica (C-a) appears rather considerable. Poor discrimination between C and Ca chromosomes supports the idea of low structural modifications of both genomes since the C. arabica speciation, at least in the frequency and distribution of repetitive sequences. GISH was also used to identify alien chromatin segments on chromosome spreads of a C liberica-introgressed line of C arabica. Further, use of GISH together with BAC-FISH analysis gave us additional valuable information about the physical localization of the C. liberica fragments carrying the S(H)3 factor involved in resistance to the coffee leaf rust. Overall, our results illustrate that FISH analysis is a complementary tool for molecular cytogenetic studies in coffee, providing rapid localization of either specific chromosomes or alien chromatin in introgressed genotypes derived from diploid species displaying substantial genomic differentiation from C. arabica.
Type de document :
Article dans une revue
Genome, NRC Research Press, 2007, 50 (7), pp.619-626. 〈10.1139/G07-034〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

http://hal.ird.fr/ird-00194793
Contributeur : Anne-Sophie Archambeau <>
Soumis le : vendredi 7 décembre 2007 - 14:28:29
Dernière modification le : mercredi 11 octobre 2017 - 01:17:33
Document(s) archivé(s) le : lundi 12 avril 2010 - 06:35:11

Fichier

Genome_JC_FISH_2007-1.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

J. C. Herrera, A. D' Hont, Philippe Lashermes. Use of fluorescence in situ hybridization as a tool for introgression analysis and chromosome identification in coffee (Coffea arabica L.). Genome, NRC Research Press, 2007, 50 (7), pp.619-626. 〈10.1139/G07-034〉. 〈ird-00194793〉

Partager

Métriques

Consultations de la notice

504

Téléchargements de fichiers

343