Alternative mutational pathways, outside the VPg, of Rice yellow mottle virus to overcome eIF(iso)4G-mediated rice resistance under strong genetic constraints

Abstract : The adaptation of rice yellow mottle virus (RYMV) to rymv1-mediated resistance has been reported to involve mutations in the viral genome-linked protein (VPg). In this study, we analysed several cases of rymv1-2 resistance breakdown by an isolate with low adaptability. Surprisingly, in these rarely occurring resistance-breaking (RB) genotypes, mutations were detected outside the VPg, in the ORF2a/ORF2b overlapping region. The causal role of three mutations associated with rymv1-2 resistance breakdown was validated via directed mutagenesis of an infectious clone. In resistant plants, these mutations increased viral accumulation as efficiently as suboptimal RB mutations in the VPg. Interestingly, these mutations are located in a highly conserved, but unfolded, domain. Altogether, our results indicate that under strong genetic constraints, a priori unfit genotypes can follow alternative mutational pathways, i.e. outside the VPg, to overcome rymv1-2 resistance.
Type de document :
Article dans une revue
Journal of General Virology, Microbiology Society, 2014, 95 (1), pp.219-224
Liste complète des métadonnées

http://hal.ird.fr/ird-01521875
Contributeur : Eugénie Hébrard <>
Soumis le : vendredi 12 mai 2017 - 14:23:55
Dernière modification le : mardi 10 octobre 2017 - 16:16:24
Document(s) archivé(s) le : dimanche 13 août 2017 - 12:43:11

Fichier

Poulicard_edited_2014.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Nils Poulicard, Agnès Pinel-Galzi, Denis Fargette, Eugénie Hébrard. Alternative mutational pathways, outside the VPg, of Rice yellow mottle virus to overcome eIF(iso)4G-mediated rice resistance under strong genetic constraints. Journal of General Virology, Microbiology Society, 2014, 95 (1), pp.219-224. 〈ird-01521875〉

Partager

Métriques

Consultations de la notice

30

Téléchargements de fichiers

53