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TERAHERTZ BIOMEMS FOR ENZYMATIC CATALYSIS MONITORING

Abdennour Abbas 1, 2, 3, * Anthony Trezeibre 1 Nourredine Bourzgui 1 Didier Guillochon 2 Dominique Vercaigne-Marko 2 Philippe Supiot 3 Bertrand Bocquet 1
* Corresponding author
1 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
2 ProBioGEM - Procédés Biologiques, Génie Enzymatique et Microbien - EA1026
3 GéPIFRéM - Laboratoire de Génie des Procédés d'Interactions Fluides Réactifs-Matériaux
Abstract : This work focuses on the fabrication and use of a Biological Micro-ElectroMechanical System (BioMEMS) for TeraHertz (THz) detection and characterization of enzymatic catalysis reactions. The fluidic microdevice was fabricated using traditional lithographic techniques. It was then functionalized by reactive amine functions using plasma polymerized allylamine (pp-allylamine), followed by enzyme immobilization inside the microchannels. The enzymatic reaction was controlled by fluorescent microscopy before being analysed by sub-THz spectroscopy in the frequency range 0.06–0.11 THz. The preliminary results show the feasibility of terahertz monitoring of the biocatalysis reaction.
Keywords : BioMEMS Sub-TeraHertz spectroscopy Enzyme reaction plasma polymer
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https://hal.ird.fr/ird-00397688
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Submitted on : Wednesday, June 24, 2009 - 1:13:48 PM
Last modification on : Monday, April 26, 2021 - 11:40:02 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 6:19:00 PM

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  • HAL Id : ird-00397688, version 1
  • ARXIV : 0907.1162

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CNRS | IRD | UNIV-LILLE | UNIV-VALENCIENNES | IEMN

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Abdennour Abbas, Anthony Trezeibre, Nourredine Bourzgui, Didier Guillochon, Dominique Vercaigne-Marko, et al.. TERAHERTZ BIOMEMS FOR ENZYMATIC CATALYSIS MONITORING. 5th International Conference on Microtechnologies in Medicine and Biology, Jun 2009, Québec City, Canada. ⟨ird-00397688⟩

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