Next generation sequencing yields complete mitogenomes of Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) (Chondrichthyes: Dasyatidae)

Kang-Ning Shen 1 Chih-Wei Chang 2 Shou-Yi Tsai 3 Shan-Chun Wu 3 Zi-Han Lin 3 Yen-Fan Chan 3 Ching-Hung Chen 4 Chung-Der Hsiao 3 Philippe Borsa 5
1 Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung
2 Department of Biology, National Museum of Marine Biology and Aquarium, Checheng
3 Chung Yuan Christian University
4 WeThink Biotech Inc., Taoyuan, Taiwan
5 ENTROPIE [Nouvelle-Calédonie] - Ecologie marine tropicale des océans Pacifique et Indien
Abstract : The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as species complexes based on morphological and molecular evidence. In this study, we used the next-generation sequencing method to decipher the two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenomes had a length of 17,690 bp in H. leoparda and 17,974 bp in N. kuhlii, and showed 78% identity to each other. Both mitogenomes had the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNAs genes and the non-coding control region. The control region, which was 1931-bp long in H. leoparda and 2243-bp long in N. kuhlii was located between the tRNA-Pro and tRNA-Phe loci. The overall GC content was 40.3% in H. leoparda and 39.8% in N. kuhlii. The complete mitogenome sequences of H. leoparda and N. kuhlii provide important molecular data for further phylogenetic and evolutionary analyses of these two stingray species complexes.
Keywords : Blue-spotted stingray next generation sequencing mitogenome Leopard whipray
Document type :
Journal articles
Complete list of metadatas

Cited literature [10 references]  Display  Hide  Download
https://hal.ird.fr/ird-01312741
Contributor : Philippe Borsa <>
Submitted on : Monday, May 9, 2016 - 1:50:09 PM
Last modification on : Tuesday, August 13, 2019 - 11:16:02 AM

File

STINGRAYS_Shen et al_Mitochond...
Files produced by the author(s)

Identifiers

Collections

IRD

Citation

Kang-Ning Shen, Chih-Wei Chang, Shou-Yi Tsai, Shan-Chun Wu, Zi-Han Lin, et al.. Next generation sequencing yields complete mitogenomes of Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) (Chondrichthyes: Dasyatidae) . Mitochondrial DNA Part A, Taylor and Francis, 2016, 27, pp.2613-2614. ⟨10.3109/19401736.2015.1041119⟩. ⟨ird-01312741v2⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

435

Files downloads

392

Contact

CCSD