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Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species

Abstract : Background : Parasite concentration methods facilitate molecular, biochemical and immunologicalresearch on the erythrocytic stages of Plasmodium. In this paper, an adaptation of magnetic MACS®columns for the purification of human Plasmodium species is presented. This method was useful forthe concentration/purification of either schizonts or gametocytes. Results and conclusions : The magnetic removal of non-parasitized red blood cells (in vivo andin vitro) using magnetic columns (MACS) was evaluated. This easy-to-use technique enrichedschizonts and gametocytes from Plasmodium falciparum in vitro cultures with a very high degree ofpurity. In addition, all haemozoin-containing stages (schizonts and/or gametocytes) from theperipheral blood of infected patients could be concentrated using this method. This method isparticularly useful for the concentration of non-falciparum species, which do not grow in cultureand are otherwise difficult to obtain in large amounts.
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Clotilde Ribaut, Antoine Berry, Severine Chevalley, Karine Reybier, Isabelle Morlais, et al.. Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species. Malaria Journal, BioMed Central, 2008, 7 (1), pp.45. ⟨10.1186/1475-2875-7-45⟩. ⟨ird-02896998⟩

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