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Isolation and characterization of microsatellites in two tropical butterflies, Drupadia theda and Arhopala epimuta (Lepidoptera: Lycaenidae)

Abstract : Little is known about the effect of El Niño Southern Oscillation-induced fires on the genetic diversity of tropical rainforest species. Here, I report on the isolation and characterization of 10 microsatellite loci, five loci each, for two lycaenid butterfly species in East Kalimantan, Indonesia, namely Drupadia theda and Arhopala epimuta , which will be used to specifically study the impact of disturbance on genetic diversity. Microsatellite enrichment was carried out using streptavidin-coated magnetic beads. Positive colonies were identified with the three-primer polymerase chain reaction (PIMA). Cross-species amplifications conducted both within and between genera were successful in 16 out of 20 tests.
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https://hal.ird.fr/ird-03044080
Contributor : Cecile Fauvelot <>
Submitted on : Monday, December 7, 2020 - 4:30:25 PM
Last modification on : Saturday, December 19, 2020 - 9:53:47 PM
Long-term archiving on: : Monday, March 8, 2021 - 7:23:47 PM

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Cécile Fauvelot. Isolation and characterization of microsatellites in two tropical butterflies, Drupadia theda and Arhopala epimuta (Lepidoptera: Lycaenidae). Molecular Ecology Notes, Wiley-Blackwell, 2005, 5, pp.724 - 726. ⟨10.1111/j.1471-8286.2005.01045.x⟩. ⟨ird-03044080⟩

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