AGO104 is a RdDM effector of paramutation at the maize b1 locus - IRD - Institut de recherche pour le développement Access content directly
Journal Articles (Data Paper) PLoS ONE Year : 2022

AGO104 is a RdDM effector of paramutation at the maize b1 locus


Although paramutation has been well-studied at a few hallmark loci involved in anthocyanin biosynthesis in maize, the cellular and molecular mechanisms underlying the phenomenon remain largely unknown. Previously described actors of paramutation encode components of the RNA-directed DNA-methylation (RdDM) pathway that participate in the biogenesis of 24-nucleotide small interfering RNAs (24-nt siRNAs) and long non-coding RNAs. In this study, we uncover an ARGONAUTE (AGO) protein as an effector of the RdDM pathway that is in charge of guiding 24-nt siRNAs to their DNA target to create de novo DNA methylation. We combined immunoprecipitation, small RNA sequencing and reverse genetics to, first, validate AGO104 as a member of the RdDM effector complex and, then, investigate its role in paramutation. We found that AGO104 binds 24-nt siRNAs involved in RdDM, including those required for paramutation at the b1 locus. We also show that the ago104-5 mutation causes a partial reversion of the paramutation phenotype at the b1 locus, revealed by intermediate pigmentation levels in stem tissues. Therefore, our results place AGO104 as a new member of the RdDM effector complex that plays a role in paramutation at the b1 locus in maize.
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Dates and versions

ird-04016415 , version 1 (30-05-2023)





Juliette Aubert, Fanny Bellegarde, Omar Oltehua-Lopez, Olivier Leblanc, Mario Arteaga-Vazquez, et al.. AGO104 is a RdDM effector of paramutation at the maize b1 locus. PLoS ONE, 2022, 17 (8), pp.e0273695. ⟨10.1371/journal.pone.0273695⟩. ⟨ird-04016415⟩
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